Description of the microscope:
Easy to use: The microscope is designed to be user-friendly, with intuitive controls and a straightforward workflow. This makes it easy to capture high-quality images quickly and efficiently. It is an excellent system to start to learn microscopy, best suited for internship students.
Routine work: The Zeiss Axio Imager M2 is designed for routine work on fixed samples, making it an ideal tool for any research labs doing microscopy imaging experiments.
Multiple imaging modes: The microscope can perform Brightfield, DIC and Epi-fluorescence imaging, giving you the flexibility to choose the imaging mode that best suits your needs.
High-quality imaging: The Zeiss Axio Imager M2 is capable of producing high-quality images with excellent contrast and resolution, thanks to its advanced imaging technology and feature like the APOTOME.
Apotome: The Apotome feature allows for structured illumination, which enables optical sectioning of fixed samples. This feature is particularly useful to produce “confocal-like” images on thin samples for a low cost compare to a confocal.
Overall, the Zeiss “APOTOME” Axio Imager M2 equipped with Apotome is an excellent tool for routine work on fixed samples. With its multiple imaging modes, high-quality imaging capabilities, and easy-to-use design, it is an ideal choice for research labs and other applications where high-quality imaging of thin sample is required.
Equipment:
Fixed samples
Tissue sections
Zeiss AxioImager M2
ref: 430004-9902-000
Upright stand
Z-drive mot. basic
stepsize 25 nm
Motorized 6-position objective revolver
ref: 424505-0000-000
Motorized 6-position reflector turret
ref: 424907-0000-000
PL 10x/23 Br.Foc.Pl
ref: 444036-9000-000
Illuminator VIS-LED
ref: 423053-9030-000
ref: 410830-1768-000
Manufacturer: Hamamatsu Photonics K.K
Catalog Number: C11440-42U30
Sensor type: Scientific CMOS
Sensor size:
18.8 mm
(13.312 mm × 13.312 mm)
Pixel count:
2048(H) × 2048(V)
= 4 MP
Pixel size:
6.5 um X 6.5 um
Bit depth:
16 bit /pixel
Exposure range:
from 3 ms to 10s
Binning: 1×1, 2×2, 4×4
Frame rate:
2048*2048: 30 fps
Quantum efficiency:
82 % at 560 nm
ref: 410902-9901-000
Window 10 pro 2004
Processor: intel(R) Xeon(R)
CPU: E5-2623 v3 @3.00 GHz
RAM: 32.0 GB
Screen: LCD TFT 24” z24n monitor
Zen module Z stack
Zen SW driver Hamamatsu camera
Screening
Phenotyping
3D Imaging
Optical sectioning
ref: 420340-9901-000
Immersion: Dry
Collar: none
Coverslip Thickness: 0.17 mm
Working Distance: 5.2 mm
Field of View: 25mm
DIC slider: EC PN 10x/0.30 I
Tube Length: infinity
ref: 420650-9901-000
Immersion: Dry
Collar: none
Coverslip Thickness: 0.17 mm
Working Distance: 0.55 mm
Field of View : 25 mm
DIC slider: EC PA 20x/0.80 II
Tube Length: infinity
ref: 420360-9900-000
Immersion: Dry
Collar: none
Coverslip Thickness: 0.17 mm
Working Distance: 0.71 mm
DIC slider: none
Field of View: 25 mm
Tube Length: infinity
ref: 420460-9900-000
Immersion: Oil
Collar: none
Coverslip Thickness: 0.17 mm
Working Distance: 0.21 mm
DIC slider: EC PN 40X/1.30 III
Field of View: 25 mm Tube Length: infinity
ref: 420782-9900-000
Immersion: Oil
Collar: none
Coverslip Thickness: 0.17 mm
Working Distance: 0.19 mm
Field of View: 25 mm
DIC slider: PA 63x/1.40 III
Tube Length: infinity
ref: 424201-9903-000
NA: 0.9
Correction: :achromatic-aplanatic
WD: 1.2 mm
6 positions: H, Ph1, Ph2, Ph3, DIC, DIC
Motorized with motorized front lens, aperture diaphragm and turret disk
Analyzer module DIC ACR P&C shift free for transmitted light
ref: 424932-9901-000
Condenser module DIC II/0.9 with polarizer
ref: 426702-0000-000
Condenser module DIC III/0.9 with polarizer
ref: 426703-0000-000
DIC slider EC PN 10x/0.30 I, 20x/0.50 II, PA 20x/0.80 II
ref: 426940-0000-000
DIC slider EC PN and Fluar 40x/1.30 III, PA 40x/0.95 III
ref: 426944-0000-000
DIC slider PA 63x/1.40 III
ref: 426957-0000-000
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X-Cite 120 LED Boost
ref: 410842-2098-000
Manufacturer: Excelitas Technologies
Catalog Number: XT120L
ref: 488049-9901-000
Excitation: Zeiss G 365
Mirror: Zeiss FT 395
Emission: Zeiss BP 445/50
ref: 000000-1031-346
Excitation: Zeiss BP 470/40
Mirror: Zeiss FT 495
Emission: Zeiss BP 525/50
ref: 489063-0000-000
Excitation: Zeiss BP 572/25 (HE)
Mirror: Zeiss FT 590 (HE)
Emission: Zeiss BP 629/62 (HE)
ref: 488050-9901-000
Excitation: Zeiss BP 640/30
Mirror: Zeiss FT 660
Emission: Zeiss BP 690/50
ref: 424931-0000-000
Mechanical stage 75×50/240°R
ref: 432001-9902-000
Specimen holder 76×26, one-hand operation for immersion
ref: 432308-0000-000
Apotome 2 for Axio Imager
ref: 423667-9100-000
Rigor and Reproducibility:
Optical configurations :
Contact fbonnet@mdibl.org today to learn more about our services and how we can help you achieve your research goals. We look forward to working with you and unlocking the mysteries of the microscopic world together!