Zeiss Apotome

Description of the microscope:

Easy to use: The microscope is designed to be user-friendly, with intuitive controls and a straightforward workflow. This makes it easy to capture high-quality images quickly and efficiently. It is an excellent system to start to learn microscopy, best suited for internship students.

Routine work: The Zeiss Axio Imager M2 is designed for routine work on fixed samples, making it an ideal tool for any research labs doing microscopy imaging experiments.

Multiple imaging modes: The microscope can perform Brightfield, DIC and Epi-fluorescence imaging, giving you the flexibility to choose the imaging mode that best suits your needs.

High-quality imaging: The Zeiss Axio Imager M2 is capable of producing high-quality images with excellent contrast and resolution, thanks to its advanced imaging technology and feature like the APOTOME.

Apotome: The Apotome feature allows for structured illumination, which enables optical sectioning of fixed samples. This feature is particularly useful to produce “confocal-like” images on thin samples for a low cost compare to a confocal.

Overall, the Zeiss “APOTOME” Axio Imager M2 equipped with Apotome is an excellent tool for routine work on fixed samples. With its multiple imaging modes, high-quality imaging capabilities, and easy-to-use design, it is an ideal choice for research labs and other applications where high-quality imaging of thin sample is required.


Suitable for Specimen

Fixed samples

Tissue sections

Microscope Stand

Zeiss AxioImager M2
ref: 430004-9902-000

Upright stand

Z-drive mot. basic
stepsize 25 nm

Motorized 6-position objective revolver
ref: 424505-0000-000

Motorized 6-position reflector turret
ref: 424907-0000-000


PL 10x/23 Br.Foc.Pl
ref: 444036-9000-000

Transmitted Illumination

Illuminator VIS-LED
ref: 423053-9030-000

Hamamatsu ORCA FLASH 4.0 LT Plus

ref: 410830-1768-000

Manufacturer: Hamamatsu Photonics K.K

Catalog Number: C11440-42U30

Sensor type: Scientific CMOS

Sensor size:
18.8 mm
(13.312 mm × 13.312 mm)

Pixel count:
2048(H) × 2048(V)
= 4 MP

Pixel size:
6.5 um X 6.5 um

Bit depth:
16 bit /pixel

Exposure range:
from 3 ms to 10s

Binning: 1×1, 2×2, 4×4

Frame rate:
2048*2048: 30 fps

Quantum efficiency:
82 % at 560 nm

Microscopy workstation compact Z840

ref: 410902-9901-000

Window 10 pro 2004

Processor: intel(R) Xeon(R)

CPU: E5-2623 v3 @3.00 GHz

RAM: 32.0 GB

Screen: LCD TFT 24” z24n monitor

Zen Pro 3.1

Zen module Z stack

Zen SW driver Hamamatsu camera

Suitable for Techniques



3D Imaging

Optical sectioning

Olympus Objectives
EC Plan-Neofluar 10x/0.3 M27

ref: 420340-9901-000

Immersion: Dry

Collar: none

Coverslip Thickness: 0.17 mm

Working Distance: 5.2 mm

Field of View: 25mm

DIC slider: EC PN 10x/0.30 I

Tube Length: infinity

Plan-Apochromat 20x/0.8 M27

ref: 420650-9901-000

Immersion: Dry

Collar: none

Coverslip Thickness: 0.17 mm

Working Distance: 0.55 mm

Field of View : 25 mm

DIC slider: EC PA 20x/0.80 II

Tube Length: infinity

EC Plan-Neofluar 40x/0.75 M27

ref: 420360-9900-000

Immersion: Dry

Collar: none

Coverslip Thickness: 0.17 mm

Working Distance: 0.71 mm

DIC slider: none

Field of View: 25 mm

Tube Length: infinity

EC Plan-Neofluar 40x/1.30 Oil M27

ref: 420460-9900-000

Immersion: Oil

Collar: none

Coverslip Thickness: 0.17 mm

Working Distance: 0.21 mm

DIC slider: EC PN 40X/1.30 III

Field of View: 25 mm Tube Length: infinity

Plan-Apochromat 63x/1.4 Oil DIC M27

ref: 420782-9900-000

Immersion: Oil

Collar: none

Coverslip Thickness: 0.17 mm

Working Distance: 0.19 mm

Field of View: 25 mm

DIC slider: PA 63x/1.40 III

Tube Length: infinity

Bright field imaging
Universal Condenser achromatic-aplanatic 0.9 H D Ph DIC, mot.

ref: 424201-9903-000

NA: 0.9

Correction: :achromatic-aplanatic

WD: 1.2 mm

6 positions: H, Ph1, Ph2, Ph3, DIC, DIC

Motorized with motorized front lens, aperture diaphragm and turret disk

DIC accessories

Analyzer module DIC ACR P&C shift free for transmitted light
ref: 424932-9901-000

Condenser module DIC II/0.9 with polarizer
ref: 426702-0000-000

Condenser module DIC III/0.9 with polarizer
ref: 426703-0000-000

DIC slider EC PN 10x/0.30 I, 20x/0.50 II, PA 20x/0.80 II
ref: 426940-0000-000

DIC slider EC PN and Fluar 40x/1.30 III, PA 40x/0.95 III
ref: 426944-0000-000

DIC slider PA 63x/1.40 III
ref: 426957-0000-000

Accordion item 3

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X-Cite 120 LED Boost
ref: 410842-2098-000

Manufacturer: Excelitas Technologies
Catalog Number: XT120L

Filter sets
DAPI – filter set 49

ref: 488049-9901-000

Excitation: Zeiss G 365
Mirror: Zeiss FT 395
Emission: Zeiss BP 445/50

eGFP – filter set 38

ref: 000000-1031-346

Excitation: Zeiss BP 470/40
Mirror: Zeiss FT 495
Emission: Zeiss BP 525/50

mCherry – filter set 63 HE

ref: 489063-0000-000

Excitation: Zeiss BP 572/25 (HE)
Mirror: Zeiss FT 590 (HE)
Emission: Zeiss BP 629/62 (HE)

Cy 5 – filter set 50

ref: 488050-9901-000

Excitation: Zeiss BP 640/30
Mirror: Zeiss FT 660
Emission: Zeiss BP 690/50

Reflector module FL EC P&C

ref: 424931-0000-000


Mechanical stage 75×50/240°R
ref: 432001-9902-000

Specimen holder 76×26, one-hand operation for immersion
ref: 432308-0000-000

Optical sectioning

Apotome 2 for Axio Imager
ref: 423667-9100-000

Rigor and Reproducibility:


Optical configurations :

Contact fbonnet@mdibl.org today to learn more about our services and how we can help you achieve your research goals. We look forward to working with you and unlocking the mysteries of the microscopic world together!