The mission of the MDIBL Light Microscopy Facility (LMF) is to provide professional scientific expertise in light microscopy. Our microscopes are available 24/7 to scientists at MDIBL and the Maine INBRE network and their collaborators, as well to summer students and visiting scientists to help them answer their research questions. Our facility is also available for educational purposes for both undergraduate and postgraduate students working at the institute or undertaking courses hosted on the MDIBL campus. Access to state-of-the-art infrastructure is essential for successful scientific research.
We offer access to cutting-edge hardware and software as well as expert guidance at any step of imaging project, from experimental design to image analysis, to provide the best service possible for our users.
If you would like to become a registered user, please check the “booking system” tab.
- Information and advice on imaging techniques and experimental design
- Instrument training: from basic use through advanced techniques
- Training in post-acquisition analysis – Imaris and Fiji
- Ongoing tailored advice and support for imaging projects
- Imaging and Image analysis service
- Bright-field and multi-channel fluorescence microscopy
- Time lapse bright-field and fluorescence imaging of live specimens.
- Laser scanning confocal microscopy, including spectral imaging, multi-positional imaging and photo-manipulation
- Spinning disk confocal imaging for live experiments in organisms, tissues or cells
- Super-resolution imaging using Airy scan 2
- Two-photon for deep tissue imaging and Second-harmonic generation imaging microscopy
- High-throughput screening of tissue and fluorescence in multi-well plates and dishes
- Image processing and segmentation with Imaris and Fiji
LMF MDIBL comprises eight microscope systems. Two laser scanning confocal microscopes: one Olympus inverted confocal microscope system (FV1000) and one Zeiss inverted confocal microscope system (LSM-980) equipped with Airy scan 2 for super resolution and 2-photon technology for in-vivo deep imaging with a temperature controlled chamber. One spinning disk confocal: a Nikon inverted spinning disk microscope system with an incubation chamber (Eclipse Ti with Yokogawa disk CSU-W1). The confocal systems are mainly used for epifluorescence imaging but also can be used for photomanipulation experiments or spectral unmixing. The facility counts also two Zeiss widefield microscopes for brightfield and epifluorescence illumination (Zeiss Apotome and Zeiss Colibri).
In addition, the LMF MDIBL comprises three macroscopes systems / zoom microscopes to observe large samples or complete model organisms in brightfield and epifluorescence: one Olympus stereomicroscope system (MVX10) and two Zeiss stereomicroscope systems (SteREO Discovery V12), fully automated and one AxioZoom V16 , fully automated with an ApoTome attachment). You benefit from quick-and-easy zooming from an overview to small details without changing the objective. Our MVX10 Olympus stereomicroscope system is manual and allows quick selection of fluorescent embryos. For brightfield or fluorescence imaging of whole organisms, the zoom stereomicroscopeSteREO Discovery.V12 and Axio Zoom.V16 are your ideal tools. They are equipped with motorized zoom, allowing you to choose the perfect magnification, while observing large samples with an enhanced three-dimensional impression. With higher apertures they achieve brighter fluorescence and higher resolution compare to conventional stereo microscopes. You even have the option to extend the Axio Zoom.V16 withApoTome.2and perform optical sectioning of your large samples.