Technical capabilities and specifications

The Light Microscopy Facility at MDIBL is equipped with advanced instrumentation and cutting-edge technology to provide high-quality microscopy services to our users. Our facility offers a range of imaging modalities and techniques, including:

1- Stereoscope macroscopes: We have two stereoscopes macroscopes, the zeiss Aiozoom V16 and an Olympus MVX10, which offer a friendly user experience to screen big samples in bright field and fluorescence. The MVX10 is fully manual, whereas the AxioZoom V16 is fully motorized allowing automatic acquisitions as well as tilling and stitching and multi position imaging.

Example of multi-positioning and automatic acquisition workflow of zebrafish embryo with the Zeiss AxioZoom V16 ©MDIBL

2- Widefield Microscopy: We have several widefield microscopes, including the Zeiss Axio Observer Z1 and the Zeiss Imager M2, which offer high-resolution imaging in both fluorescence and brightfield modes. These microscopes are equipped with high-sensitivity cameras either monochrome for fluorescence imaging or color camera to image histological samples. They are equipped with incubation chamber for live cell experiments a well optical sectioning for “confocal-like” imaging. Both offer motorized stages for automated imaging.

Example of High-throughput imaging on our Zeiss “colibri” ©MDIBL

3-Confocal Microscopy: We have multiple confocal microscopes, including the Zeiss LSM 980 and the Nikon point scanning C2+ / spinning disk CSU-W1 which offer high-resolution imaging of fluorescently labeled samples. These microscopes are equipped with a range of objectives, from 4x to 63x, and can perform different advanced techniques such as spectral imaging, Fluorescence Recovery After Photobleaching (FRAP) and automatic acquisition.

Sceenshot of the JOBS module on a Nikon Confocal fo automatic 384 well plate imaging and analysis ©ucsd

4- Super-Resolution Microscopy: Our facility offers super-resolution microscopy services using the Zeiss 980 with airy scan 2 detector, which provide resolution beyond the diffraction limit of traditional microscopy techniques. This microscopes enable imaging of sub-cellular structures and dynamics with ultra-high resolution.

Comparing the confocal image (left) with Airyscan SR (middle) and Airyscan Joint Deconvolution (right). HeLa cell, 4x expanded and labelled with acetylated alpha tubulin (green) ©Zeiss

5-Multi Photon microscopy: Our facility offers multi photon microscopy services using the Zeiss 980 with a tunable Coherent Chameleon Vision 2 laser. GaAsP non de-scanned detector, quasar 34 detection channels or airy scan 2 detector can be used to provide the best quality image for deep tissue imaging.

6-Light-sheet microscopy: Our facility offers light-sheet microscopy with the mesoSPIM (“mesoscale selective plane-illumination microscopy”) microscope that is a state-of-the-art imaging technique that enables researchers to capture high-resolution 3D images of biological specimens with unprecedented speed and precision. The mesoSPIM microscope uses a combination of selective plane illumination and light-sheet fluorescence microscopy to provide a fast and gentle way to image samples that are too thick or too large to be imaged with traditional microscopy methods. This innovative imaging technology has a wide range of applications in neuroscience, developmental biology, and other fields of research, where it allows scientists to study the inner workings of tissues-cleared organisms in exquisite detail.

Whole mouse brain cleared during an imaging session with the MesoSpim ©MDIBL

7-Image Analysis: Our facility offers a range of software solutions for image analysis, including FIJI (ImageJ), Imaris, and Huygens. We have dedicated workstations for data analysis, as well as staff with expertise in quantitative analysis, colocalization studies, and image processing.

Example of image segmentation using artifficial intelligence (LabKit)

8-Live Cell Imaging: Our facility offers live cell imaging capabilities with temperature and CO2 control, enabling researchers to study cellular processes in real-time.

zebrafish embryo calcium imaging using the nikon sniping disk

Contact fbonnet@mdibl.org today to learn more about our services and how we can help you achieve your research goals. We look forward to working with you and unlocking the mysteries of the microscopic world together!