The mesoSPIM

Description of the microscope:

Flexibility and versatility: The MesoSPIM is a cutting-edge light-sheet microscope that addresses the challenges of imaging large cleared samples with near-isotropic resolution in a matter of minutes. It is an open-hardware project that is compatible with any type of cleared or expanded sample. Unlike many existing instruments, the MesoSPIM is designed with large samples in mind (mm) and can accommodate even whole mouse central nervous systems without cutting. The instrument combines a large imaging volume, excellent image quality over large fields-of-view, and simple and versatile sample handling.

Features : The MesoSpim is the ultimate tool for high-speed, isotropic imaging of cleared samples.

With a scanning rate of 5-8 minutes, this axially scanned light-sheet microscope provides an uniform 6.5um voxel size across the entire field of view, ensuring exceptional image quality. With a vertical sample rotation axis, you can rotate your sample 360° without changing the direction of gravity, preventing rotation-induced distortions of soft samples.

Sample mounting is made easy with the kinematic mounts and magnetic sample holders that allow for fast sample exchange. Thanks to the different corvette sizes and its large travel range (44.5 mm × 44.5 mm × 100 mm), the mesoSPIM is compatible with a wide range of sample clearing techniques, including CLARITY, X-CLARITY, CUBIC-X, BABB, iDISCO, and MASH and sizes. This makes it ideal for screening batches of cleared samples, saving valuable time and effort.

The mesoSPIM’s excitation path (Dual-sided illumination and the Axially scanned light-sheet technology) is designed to reduce shadowing artifacts, producing a uniform illumination profile in the sample that simplifies data analysis.

Easy of use: The mesoSPIM is designed to be user-friendly, with simple sample mounting and fast acquisitions that allow for quick screening of samples.

Full suite of software for image processing and analysis: To facilitate image processing and analysis, several software package come with the microscopes like IMARIS, Huygens, AMIRA and Wiener.

Overall, the mesoSPIM is an easy-to-use microscope that provides isotropic data from a cleared sample within 5-8 minutes, making it a valuable tool for researchers in neuroscience and beyond.


Suitable for Specimen

Cleared samples

Whole organisms or organs

Microscope Stand

Olympus MVX-ZB10
ref: N1564600

Horizontal detection

Ludl Motorized 10 pos Filter Wheel
ref: 96A350



Kinetix Scientific CMOS (sCMOS)

ref: 410830-1768-000

Manufacturer: Teledyne Photometrics

Sensor type: Scientific CMOS

Sensor size:
29.4mm diagonal
(20.8mm x 20.8mm)

Pixel count:
3200(H) x 3200(V)
= 10.24 MP

Pixel size:
6.5μm x 6.5μm

Bit depth:
8 (speed)-12 (sensitivity)-16 bit (dynamic range)/pixel

Frame rate:
5.2 fps to 498fps

Quantum efficiency:
96 % at 600 nm


Microscopy workstation —

ref: 410902-9901-000

Window 10 pro 2004

Processor: intel(R) Xeon(R)

CPU: E5-2623 v3 @3.00 GHz

RAM: 32.0 GB

Screen: LCD TFT 24” z24n monitor




Tilling and stitching

multi-color imaging

Time-lapse imaging

Suitable for Techniques



3D Imaging

Optical sectioning

Tilling and stiching

Miltenyi-Biotec Detection Objective
MI Plan 1.1X/0.1NA

Ref: 130-133-611

Working distance: <17mm

MI Plan 4.0X/0.35NA

Ref: 130-133-602

Working distance: <16mm

MI Plan 12X/0.53NA

Ref: 130-133-605

Working distance: <10.9mm

Light sheet
Laser unit

– 405nm – 50 mW
laser head

– 488nm – 60 mW
laser head

– 561nm – 35 mW
laser head

– 594nm – 50mW
laser head

– 647nm – 50mW
laser head

Electrically Tunable Lens
EL16 Lenses


ref: EL-16-40-TC-VIS-5D-1-C

Lens Driver


ref: EL-E-4i




Emission filters

ref: Chroma ZET405/488/561/647m

DAPI: 450/60

EFPG: 525/50

AF555: 630/27



ref: Chroma ET480/30m


ref: Chroma ET540/41m

custom design

AF 555-SB

ref: Chroma D580/30m


ref: Chroma AT605/30m

AF 647_LP1

ref: Chroma ET660/lp

custom design

AF 647_LP2

ref: Chroma ET680/lp

custom design

Sample cuvettes

Portmann Instruments


Rigor and Reproducibility:


Optical configurations :

Contact today to learn more about our services and how we can help you achieve your research goals. We look forward to working with you and unlocking the mysteries of the microscopic world together!