Why acknowledging core facilities is important ?
Contributions to scientific output: scientific papers, posters, Bachelor-, Master- or doctoral theses and conferences, is key metric for core facility management, as it allows to get funding, grants, prove the impact of the facility, prove the effective use of the equipment and to ensure continuous high standards of operations. That is why acknowledging a core facility in publications is an important matter.

Guidelines for Acknowledgments and Authorship:
All publications or any data acquired or processed using LMF MDIBL equipment and/or services must be acknowledged in every publication as follows:

“ Image collection, processing and analysis for this manuscript was performed with the assistance of Dr. Frederic Bonnet and the MDI Biological Laboratory Light Microscopy Facility, which is supported by the Maine INBRE grant (GM103423) from the NIGMS at the NIH.

The Royal Microscopy Society (RMS) has released a generic Core Facilities Publication Policy with baseline recommendations to acknowledge Core facility staff in the appropriate way:

Acknowledgement of facility contributions is expected in publications that include any data generated in the facility. For example, when fee-for-service is performed with no method development and minimum effort by Core personnel.

Co-authorship is generally expected when core personnel have made assistance above the technical or routine level, with any facility staff providing scientific input and expertise in experimental set-up, method development, data acquisition and/or analysis and/or interpretation. Significant can be defined as “the project would not have progressed, or progressed at a substantially slower pace, without the guidance of facility personnel”. In such cases, core personnel should have the opportunity to review and edit the appropriate sections of a manuscript before submission.

Please discuss acknowledgments with facility staff prior to manuscript submission.

Source: Royal Microscopical Society and BioImaging UK

Good research practices: rigor and reproducibility

Microscopy techniques become more powerful and more complex, it is vital that scientific articles containing images obtained with advanced microscopes include full details about how each image was obtained. A recent survey (see : Imaging methods are vastly under reported in biomedical research) shows that the quality of reporting was poor, with some articles containing no information about how images were obtained, and many articles lacking important basic details.

To improve the quality of the research done at the LMF @MDIBL , by making sure that the results are presented optimally and comply with best research practices, all the users are asked to contact the facility staff to review the sections describing the microscopy methods in drafts of publications.

After publication, facility users are requested to provide a PDF-file of the publication for the facility’s records.



Adamla F, Rollins J, Newsom M, Snow S, Schosserer M, Heissenberger C, Horrocks J, Rogers AN, Ignatova Z. A Novel Caenorhabditis Elegans Proteinopathy Model Shows Changes in mRNA Translational Frameshifting During Aging. Cell Physiol Biochem [Internet]. 2019;52(5):970–983.

Grendler J, Lowgren S, Mills M, Losick VP. Wound-induced polyploidization is driven by Myc and supports tissue repair in the presence of DNA damage. Development [Internet]. 2019;146(15).

Lan J, Rollins JA, Zang X, Wu D, Zou L, Wang Z, Ye C, Wu Z, Kapahi P, Rogers AN, Chen D. Translational Regulation of Non-autonomous Mitochondrial Stress Response Promotes Longevity. Cell Rep [Internet]. 2019;28(4):1050-1062.e6.

Mahringer A, Bernd A, Miller DS, Fricker G. Aryl hydrocarbon receptor ligands increase ABC transporter activity and protein expression in killifish (Fundulus heteroclitus) renal proximal tubules. Biol Chem [Internet]. 2019;400(10):1335–1345.

Marnik EA, Fuqua JH, Sharp CS, Rochester JD, Xu EL, Holbrook SE, Updike DL. Germline Maintenance Through the Multifaceted Activities of GLH/Vasa in Caenorhabditis elegans P Granules. Genetics [Internet]. 2019;213(3):923–939.

Müller-Deile J, Schenk H, Schroder P, Schulze K, Bolaños-Palmieri P, Siegerist F, Endlich N, Haller H, Schiffer M. Circulating factors cause proteinuria in parabiotic zebrafish. Kidney Int [Internet]. 2019;96(2):342–349.

Müller-Deile J, Schenk H, Niggemann P, Bolaños-Palmieri P, Teng B, Higgs A, Staggs L, Haller H, Schroder P, Schiffer M. Mutation of microphthalmia-associated transcription factor (mitf) in zebrafish sensitizes for glomerulopathy. Biol Open [Internet]. 2019 Mar 4;8(3).

Schenk H, Müller-Deile J, Schroder P, Bolaños-Palmieri P, Beverly-Staggs L, White R, Bräsen JH, Haller H, Schiffer M. Characterizing renal involvement in Hermansky-Pudlak Syndrome in a zebrafish model. Sci Rep [Internet]. 2019;9(1):17718.

Schenk H, Masseli A, Schroder P, Bolaños-Palmieri P, Beese M, Hegermann J, Bräsen JH, Haller H. Sulfatases, in Particular Sulf1, Are Important for the Integrity of the Glomerular Filtration Barrier in Zebrafish. Biomed Res Int [Internet]. 2019;2019:4508048.

Smith AM, Dykeman CA, King BL, Yin VP. Modulation of TNFα Activity by the microRNA Let-7 Coordinates Zebrafish Heart Regeneration. iScience [Internet]. 2019 May 31;15:1–15.

Tossidou I, Teng B, Worthmann K, Müller-Deile J, Jobst-Schwan T, Kardinal C, Schroder P, Bolanos-Palmieri P, Haller H, Willerding J, Drost DM, de Jonge L, Reubold T, Eschenburg S, Johnson RI, Schiffer M. Tyrosine Phosphorylation of CD2AP Affects Stability of the Slit Diaphragm Complex. J Am Soc Nephrol [Internet]. 2019;30(7):1220–1237.

Beck S, Rhee C, Song J, Lee B-K, LeBlanc L, Cannon L, Kim J.”> Implications of CpG islands on chromosomal architectures and modes of global gene regulation. Nucleic Acids Res [Internet]. 2018;46(9):4382–4391.

Hersh TA, Dimond AL, Ruth BA, Lupica N V, Bruce JC, Kelley JM, King BL, Lutton B V. A role for the CXCR4-CXCL12 axis in the little skate, Leucoraja erinacea. Am J Physiol Regul Integr Comp Physiol [Internet]. 2018;315(2):R218–R229.

Müller-Deile J, Schröder P, Beverly-Staggs L, Hiss R, Fiedler J, Nyström J, Thum T, Haller H, Schiffer M. Overexpression of preeclampsia induced microRNA-26a-5p leads to proteinuria in zebrafish. Sci Rep [Internet]. 2018;8(1):3621.

Neuman RI, van Kalmthout JAM, Pfau DJ, Menendez DM, Young LH, Forrest JN. AMP-activated protein kinase and adenosine are both metabolic modulators that regulate chloride secretion in the shark rectal gland ( Squalus acanthias). Am J Physiol Cell Physiol [Internet]. 2018;314(4):C473–C482.

Rinschen MM, Gödel M, Grahammer F, Zschiedrich S, Helmstädter M, Kretz O, Zarei M, Braun DA, Dittrich S, Pahmeyer C, Schroder P, Teetzen C, Gee H, Daouk G, Pohl M, Kuhn E, Schermer B, Küttner V, Boerries M, Busch H, Schiffer M, Bergmann C, Krüger M, Hildebrandt F, Dengjel J, Benzing T, Huber TB. A Multi-layered Quantitative In Vivo Expression Atlas of the Podocyte Unravels Kidney Disease Candidate Genes. Cell Rep [Internet]. 2018;23(8):2495–2508.

Waldron AL, Schroder PA, Bourgon KL, Bolduc JK, Miller JL, Pellegrini AD, Dubois AL, Blaszkiewicz M, Townsend KL, Rieger S. Oxidative stress-dependent MMP-13 activity underlies glucose neurotoxicity. J Diabetes Complications [Internet]. 2018;32(3):249–257.

Yasuda-Yamahara M, Rogg M, Frimmel J, Trachte P, Helmstaedter M, Schroder P, Schiffer M, Schell C, Huber TB. FERMT2 links cortical actin structures, plasma membrane tension and focal adhesion function to stabilize podocyte morphology. Matrix Biol [Internet]. 2018;68–69:263–279.

Yin VP. In Situ Detection of MicroRNA Expression with RNAscope Probes. Methods Mol Biol [Internet]. 2018;1649:197–208.

Andralojc KM, Campbell AC, Kelly AL, Terrey M, Tanner PC, Gans IM, Senter-Zapata MJ, Khokhar ES, Updike DL. ELLI-1, a novel germline protein, modulates RNAi activity and P-granule accumulation in Caenorhabditis elegans. PLoS Genet [Internet]. 2017;13(2):e1006611.

Duong M, Yu X, Teng B, Schroder P, Haller H, Eschenburg S, Schiffer M. Protein kinase C ϵ stabilizes β-catenin and regulates its subcellular localization in podocytes. J Biol Chem [Internet]. 2017;292(29):12100–12110.

Godwin JW, Debuque R, Salimova E, Rosenthal NA. Heart regeneration in the salamander relies on macrophage-mediated control of fibroblast activation and the extracellular landscape. NPJ Regen Med [Internet]. 2017;2.

Lisse TS, Rieger S. IKKα regulates human keratinocyte migration through surveillance of the redox environment. J Cell Sci [Internet]. 2017;130(5):975–988.

Müller-Deile J, Dannenberg J, Schroder P, Lin M-H, Miner JH, Chen R, Bräsen J-H, Thum T, Nyström J, Staggs LB, Haller H, Fiedler J, Lorenzen JM, Schiffer M. Podocytes regulate the glomerular basement membrane protein nephronectin by means of miR-378a-3p in glomerular diseases. Kidney Int [Internet]. 2017;92(4):836–849.

Rochester JD, Tanner PC, Sharp CS, Andralojc KM, Updike DL. PQN-75 is expressed in the pharyngeal gland cells of Caenorhabditiselegans and is dispensable for germline development. Biol Open [Internet]. 2017 Sep 15;6(9):1355–1363.

Rollins JA, Howard AC, Dobbins SK, Washburn EH, Rogers AN. Assessing Health Span in Caenorhabditis elegans: Lessons From Short-Lived Mutants. J Gerontol A Biol Sci Med Sci [Internet]. 2017 Apr 1;72(4):473–480.

Smith AM, Maguire-Nguyen KK, Rando TA, Zasloff MA, Strange KB, Yin VP. The protein tyrosine phosphatase 1B inhibitor MSI-1436 stimulates regeneration of heart and multiple other tissues. NPJ Regen Med [Internet]. 2017;2:4.

Updike D. swt-3 transcripts are expressed in Z1/Z4 during embryogenesis and in the adult spermatheca. microPublication Biol [Internet]. 2017 Jul 20;2017.

Updike D. acdh-1 transcripts are expressed in the intestine. microPublication Biol [Internet]. 2017 Jul 20;2017.

Zaremba A, Miller DS, Fricker G. Zinc chloride rapidly stimulates efflux transporters in renal proximal tubules of killifish (Fundulus heteroclitus). Toxicol Appl Pharmacol. 2017 Nov 1;334:88-99

Bodnar AG, Coffman JA. Maintenance of somatic tissue regeneration with age in short- and long-lived species of sea urchins. Aging Cell [Internet]. 2016;15(4):778–87.

Hartig EI, Zhu S, King BL, Coffman JA. Cortisol-treated zebrafish embryos develop into pro-inflammatory adults with aberrant immune gene regulation. Biol Open [Internet]. 2016 Aug 15;5(8):1134–41.

Howard AC, Rollins J, Snow S, Castor S, Rogers AN. Reducing translation through eIF4G/IFG-1 improves survival under ER stress that depends on heat shock factor HSF-1 in Caenorhabditis elegans. Aging Cell [Internet]. 2016 Dec;15(6):1027–1038.

King BL, Shi LF, Kao P, Clusin WT. Calcium activated K+ channels in the electroreceptor of the skate confirmed by cloning. Details of subunits and splicing. Gene [Internet]. 2016 Mar 1;578(1):63–73.

King BL, Yin VP. A Conserved MicroRNA Regulatory Circuit Is Differentially Controlled during Limb/Appendage Regeneration. PLoS One [Internet]. 2016;11(6):e0157106.

Korstanje R, Deutsch K, Bolanos-Palmieri P, Hanke N, Schroder P, Staggs L, Bräsen JH, Roberts ISD, Sheehan S, Savage H, Haller H, Schiffer M. Loss of Kynurenine 3-Mono-oxygenase Causes Proteinuria. J Am Soc Nephrol [Internet]. 2016 Nov;27(11):3271–3277.

Lee EC, Kim H, Ditano J, Manion D, King BL, Strange K. Abnormal Osmotic Avoidance Behavior in C. elegans Is Associated with Increased Hypertonic Stress Resistance and Improved Proteostasis. PLoS One [Internet]. 2016;11(4):e0154156.

Lisse TS, King BL, Rieger S. Comparative transcriptomic profiling of hydrogen peroxide signaling networks in zebrafish and human keratinocytes: Implications toward conservation, migration and wound healing. Sci Rep [Internet]. 2016 Feb 5;6:20328.

Lisse TS, Middleton LJ, Pellegrini AD, Martin PB, Spaulding EL, Lopes O, Brochu EA, Carter E V, Waldron A, Rieger S. Paclitaxel-induced epithelial damage and ectopic MMP-13 expression promotes neurotoxicity in zebrafish. Proc Natl Acad Sci U S A [Internet]. 2016 Apr 12;113(15):E2189-98.

Losick VP, Jun AS, Spradling AC. Wound-Induced Polyploidization: Regulation by Hippo and JNK Signaling and Conservation in Mammals. PLoS One [Internet]. 2016;11(3):e0151251.

Rizzo F, Coffman JA, Arnone MI. An Elk transcription factor is required for Runx-dependent survival signaling in the sea urchin embryo. Dev Biol [Internet]. 2016;416(1):173–186.

Telles CJ, Decker SE, Motley WW, Peters AW, Mehr AP, Frizzell RA, Forrest JN. Functional and molecular identification of a TASK-1 potassium channel regulating chloride secretion through CFTR channels in the shark rectal gland: implications for cystic fibrosis. Am J Physiol Cell Physiol [Internet]. 2016 Dec 1;311(6):C884–C894.

Teng B, Schroder P, Müller-Deile J, Schenk H, Staggs L, Tossidou I, Dikic I, Haller H, Schiffer M. CIN85 Deficiency Prevents Nephrin Endocytosis and Proteinuria in Diabetes. Diabetes [Internet]. 2016 Dec;65(12):3667–3679.

Wu C-W, Deonarine A, Przybysz A, Strange K, Choe KP. The Skp1 Homologs SKR-1/2 Are Required for the Caenorhabditis elegans SKN-1 Antioxidant/Detoxification Response Independently of p38 MAPK. PLoS Genet [Internet]. 2016 Oct;12(10):e1006361.

Yamada T, Krzeminski M, Bozoky Z, Forman-Kay JD, Strange K. Role of CBS and Bateman Domains in Phosphorylation-Dependent Regulation of a CLC Anion Channel. Biophys J [Internet]. 2016 Nov 1;111(9):1876–1886.

Yamada T, Strange K. Regulatory Conformational Coupling between CLC Anion Channel Membrane and Cytoplasmic Domains. Biophys J [Internet]. 2016 Nov 1;111(9):1887–1896.

Yamada T, Wondergem R, Morrison R, Yin VP, Strange K. Leucine-rich repeat containing protein LRRC8A is essential for swelling-activated Cl- currents and embryonic development in zebrafish. Physiol Rep [Internet]. 2016;4(19).

Zhao H, Rieger S, Abe K, Hewison M, Lisse TS. DNA Damage-Inducible Transcript 4 Is an Innate Surveillant of Hair Follicular Stress in Vitamin D Receptor Knockout Mice and a Regulator of Wound Re-Epithelialization. Int J Mol Sci [Internet]. 2016 Nov 26;17(12).