2024 Image Contest Winners
Sweet Embryo – Travis D. Carney
Mount Desert Island Biological Laboratory
MDIBL Light Microscopy Facility
Fruit fly embryo
Sample: Drosophila embryo mounted in Vectashield
Label: Mouse anti-FasIII primary antibody + Alexa 647-conjugated secondary antibody.
Instrument: Zeiss LSM 980
Scale: Embryo is about 500 x 280 microns in size.
Image or Sample Processing Steps: Color-coded projection: different focal planes are in different colors.
I love lamp – Hannah Somers
Mount Desert Island Biological Laboratory
MDIBL Light Microscopy Facility
A beautiful moth imaged using the Nikon T-ie spinning disk confocal.
Sample: A moth found in a spider web
Label: Autofluorscence was imaged.
Instrument: Reflected light, Nikon Ti-E YokoGawa CSU-W1 / Nikon C2+ using the 4x Objective 0.2 NA
Scale: 6 mm x 4 mm
Image or Sample Processing Steps: A z-stack and tiling were used to acquire the image, and finally a max intensity projection was created using the Nikon software. LUT were applied on Fiji.
2023 Image Contest Winners
Neuronal forest – Marko Pende
Mount Desert Island Biological Laboratory
MDIBL Light Microscopy Facility
Lost in the thoughts of an cortical forest
Sample: Thy1-EGFP mouse brain, Tissue-cleared with CLARITY showing cortical neurons
Label: Endogenous EGFP signal in Thy1 neurons
Instrument: Light-sheet microscopy, Custom built, 12xNA 0.53
Scale: 750umx500um
Image or Sample Processing Steps: 250um
Leaf Walker – Hannah Somers
MDI Biological Laboratory
MDIBL Light Microscopy Facility
A beautiful tardigrade crawling on a leaf.
Sample: Tardigrade
Label: Lipid stain is shown in blue, DAPI staining is shown in yellow, autofluorescence is magenta
Instrument: Fluorescent microscopy was done on the Nikon Ti-E YokoGawa CSU-W1 / Nikon C2+ using the 60x oil objective, N.A. 1.40
Scale: 1mm x 0.5 mm
Image or Sample Processing Steps: This image required z-stack and tiling, and is a maximum intensity projection.
Photo gallery 2024
Photo gallery 2023
