Flexibility and versatility: The Nikon confocal offers both spinning disk and point scanning capabilities, allowing for a wide range of imaging applications. The seven laser lines and filters also provide flexibility in terms of fluorophore selection. This makes it a valuable tool for researchers working on a variety of projects. Additionally, the stage is fully motorized and equipped with a stage-top incubator providing appropriate temperature and CO2 for live samples.
Strengths of each scanning mode: The CSU-W1 spinning disk confocal is ideal for live cell imaging or fixed samples when speed and reduced photo-toxicity are required. On the other hand, the C2+ point scanner offers the flexibility to adjust the scan zoom for optimal pixel size and resolution, while also allowing for deeper penetration into thicker samples. Overall, the large field of view and high resolution make it well-suited for slide scanning, plate scanning, tiling of large images, and live cell imaging.
Easy of use: The fact that both scanning modes can be used with the same motorized microscope, the same software and can be switched with a simple click makes the acquisition process fast and simple.
Latest software upgrades: The microscope has been upgraded with the latest software, including artificial intelligence de-noising, which can help improve the quality of images. This is a valuable feature that can appeal to researchers looking for ways to optimize image quality.
Overall, thanks to its flexibility, versatility, ease of use, and latest software upgrades, the capabilities of this Nikon confocal microscope can be optimized and promoted to researchers looking for a powerful imaging tool for their research projects.
Live samples
Fixed samples
Tissue sections
Cell cultures
Nikon Ti-Eclipse
ref: TI-E -736803
Inverted stand
Z-drive mot.
Minimum step: 0.025μm
Maximum speed: 2.5mm/sec or higher
Motorized escape and refocus mechanism (coarse)
Coarse/fine switchable
Motorized 6-position objective revolver
ref:TI-ND6-PFS
Motorized 6-position reflector turret
Perfect Focus System with PFS offset dial
CFI 10X/22
Eyepiece tube
ref:TI-TS
Eyepiece Tube Base Unit
ref:TI-T-B
IDiascopic Illumination Pillar 100W
ref: TI-DH-612504
Precentered Dia illumination lamphouse
ref: D-LH/LC-256834
V2-A LL Halogen Lamp bulb 12V 100W
Manufacturer: Oko-Lab
Heated Chamber for Nikon Motorized Stage
ref: H301-NIKON-Ti-S-ER
All in One Controller with C2 Air Mixer
ref: UNO-T-H-CO
4x 35mm Dish Holder
ref: 4×35-M
96 Well Plate Holder
ref: 96MW
#1 1×3 inch Slide and #2 35mm Dish Holder
ref: GS35-M
1×2 inch Chambered Cover Glass Holder Magnetic
ref: 1xLABTEK-M
1×3 inch Chamber Slide Holder Magnetic
ref: 2xGS-M
Open frame for multi well plates w/ oil immersion objectives
ref: MW-OIL
Model: C2+ point scanning
Manufacturer: Nikon
ref: C2-SH-42065
ref:LU-N4-SN40475
– 405nm – 15 mW
laser head
– 488nm – 15 mW
laser head
– 561nm – 15 mW
laser head
– 640nm – 15 mW
laser head
ref: C2-Du3-14487
3 confocal PMT (2 filter cubes)
– C2 V4C EM 445/35 + 660LP
ref: Chroma 99430/C171408
– C2 EM 525/50 + 600/50
ref: Chroma 99441/C172339
1 T-PMT
Ref: 77019756
Hp Z4
Intel Xeon 3.7 GHz processor (8 cores)
464GB DDR4 2666Mhz RAM
NVIDIA RTX4000 8GB
512GB m.2 Turbo SSD
1TB M.2 Turbo SSD data drive
12TB data storage
Window 10 64-bit professional
37.5 inch IPS, LED backlit monitor
Nikon NIS elements AR 5.41.02 (buid 1711)
Live Imaging
3D imaging
Multi position acquisition
Large area tile/mosaic scans
Optical sectioning
Ref: MRD 70040
Immersion: Dry
Collar: none
Coverslip Thickness: 0/0.17 mm
Working Distance: 20.0 mm
Field of View: 25mm
Tube Length: infinity
Iref: MRD 70170
Immersion: Dry
Collar: none
Coverslip Thickness: 0.17 mm
Working Distance: 4.0 mm
Field of View : 25 mm
Tube Length: infinity
ref: MRD00205
Immersion: Dry
Collar: none
Coverslip Thickness: 0.17 mm
Working Distance: 1.0 mm
DIC slider: N2
Field of View: 1.25 mm
Tube Length: infinity
Coverslip Thickness: 0.17
λ : Nano Crystal Coat
ref: MRD00405
Immersion: Dry
Collar: yes
Coverslip Thickness: 0.11-0.23 mm
Working Distance: 0.25-0.17 mm
DIC slider: M/N2
Field of View: 25 mm
Tube Length: infinity
ref: MRH01401
Immersion: Oil
Collar: no
Coverslip Thickness: 0.17 mm
Working Distance: 0.24 mm
DIC slider: N2
Field of View: 0.63 mm
Tube Length: infinity
ref: MRD71600
Immersion: Oil
Collar: none
Coverslip Thickness: 0.17 mm
Working Distance: 0.14 mm
Field of View: 0.37 mm
Nomarski Prism: N2
Tube Length: infinity
ref: MRD77410
Immersion: Water
Collar: yes
Coverslip Thickness: 0.15-0.19 mm
Working Distance: 0.61–0.59 mm
Field of View: 25 mm
Nomarski Prism: N2
Tube Length: infinity
Ref TI-C-518109
Long workind distance Condenser lens ref:TI-C-LWD
NA: 0.52
WD: 30 mm
Manual
TC-C-DICP DIC Polarizer
Ti-A Analyzer Block Installs in Epi Fluor Turret
T-C DIC Module LWD N2 Dry
T-C DIC Module LWD NR Dry
D-C DIC Slider 20
D-C DIC Slider 40x I
DIC Slider 60x I-RD-C
DIC Slider 100x II
X-Cite 120 LED Boost
Manufacturer: Excelitas Technologies
Catalog Number: XT120L
ref: Nikon 96221 / Chroma 39000
Excitation: AT375/28x
Mirror: AT415-DC
Emission: AT460/50m
ref: Nikon 96227 / Chroma 39003
Excitation: AT480/30x
Mirror: AT505DC
Emission: AT535/40m
ref: Nikon 96231 / Chroma 39010
Excitation: AT560/40x
Mirror: AT600DC
Emission: AT635/60
Motorized Stage with Encoder
Manufacturer: Nikon
ref: Ti-S-ER
Stage Joystic:
Manufacturer: Nikon
ref: TI-S-E JOY-559960
Adapter for Oko-Lab Stagetop Incubator System
ref:H301-NIKON-TI-S-ER
LUNF-XL laser combiner
ref:77098033
– 405nm – 50 mW
laser head
– 445nm – 25 mW
laser head
– 488nm – 60 mW
laser head
– 514nm – 35 mW
laser head
– 561nm – 50mW
laser head
– 594nm – 40 mW
laser head
– 640nm – 40 mW
laser head
– 405nm – 50 mW for photo-manipulation
Manufacturer: Andor
Model: Zyla 4.2
ref: ZYLA-4.2-USB3
Sensor type: Scientific CMOS
Sensor size: 13.3*13.3 mm
Pixel count: 2048(H) × 2048(V) = 4 MP
Pixel pitch: 6.5 μm (H) x 6.5 μm (V)
Bit depth: 12 bits and 16 bits
Quantum efficiency: 72% around 600nm
Max Frame rate: 128*128: 1578 fps
Model: CSU-W1 spinning-disk confocal
Manufacturer: Yokogawa
ref: CSUW1-T1N12-C10C00-F2ED
DM 405/488/561/640 dichroic beamsplitter
Manufacturer: yokogawa
ref: nikon MHE46420
DM 445/514/594 dichroic beamsplitter
Manufacturer: semrock
ref: nikon 99226
Manufacturer: semrock
BrightLine® quad-band bandpass filter – 440/521/607/700 nm
ref: FF01-440/521/607/700
Manufacturer: chroma
ET436/20x single-band bandpass filter
ref: ET436/20x
Manufacturer: semrock
482/25 nm BrightLine® single-band bandpass filter
ref: FF01-482/25
Manufacturer: semrock
525/50 nm BrightLine® single-band bandpass filter
ref: FF01-525/50
Manufacturer: Chroma
ET545/25x bandpass filter
ref: ET545/25x
Manufacturer: Chroma
ET605/52m bandpass filter
ref: ET605/52m
Manufacturer: semrock
617/73 nm BrightLine® single-band bandpass filter
ref: FF01-617/73
Manufacturer: semrock
641/75 nm BrightLine® single-band bandpass filter
ref: FF02-641/75
Manufacturer: Chroma
ET705/72m single-band bandpass filter
ref: ET705/72m
Microlens‐enhanced Nipkow disk scanning
Max 4,000 RPM
200 FPS
50um or 25um (up to 2) motorized
17x16mm maximum
MW-OIL
C2+ filter scan head
CSU-W1 emission filters
Contact fbonnet@mdibl.org today to learn more about our services and how we can help you achieve your research goals. We look forward to working with you and unlocking the mysteries of the microscopic world together!