Nikon Ti-E YokoGawa CSU-W1 / Nikon C2+

Flexibility and versatility: The Nikon confocal offers both spinning disk and point scanning capabilities, allowing for a wide range of imaging applications. The seven laser lines and filters also provide flexibility in terms of fluorophore selection. This makes it a valuable tool for researchers working on a variety of projects. Additionally, the stage is fully motorized and equipped with a stage-top incubator providing appropriate temperature and CO2 for live samples.

Strengths of each scanning mode: The CSU-W1 spinning disk confocal is ideal for live cell imaging or fixed samples when speed and reduced photo-toxicity are required. On the other hand, the C2+ point scanner offers the flexibility to adjust the scan zoom for optimal pixel size and resolution, while also allowing for deeper penetration into thicker samples. Overall, the large field of view and high resolution make it well-suited for slide scanning, plate scanning, tiling of large images, and live cell imaging.

Easy of use: The fact that both scanning modes can be used with the same motorized microscope, the same software and can be switched with a simple click makes the acquisition process fast and simple.

Latest software upgrades: The microscope has been upgraded with the latest software, including artificial intelligence de-noising, which can help improve the quality of images. This is a valuable feature that can appeal to researchers looking for ways to optimize image quality.

Overall, thanks to its flexibility, versatility, ease of use, and latest software upgrades, the capabilities of this Nikon confocal microscope can be optimized and promoted to researchers looking for a powerful imaging tool for their research projects.

Suitable for Specimen

Live samples

Fixed samples

Tissue sections

Cell cultures

Microscope Stand

Nikon Ti-Eclipse
ref: TI-E -736803

Inverted stand

Z-drive mot.
Minimum step: 0.025μm
Maximum speed: 2.5mm/sec or higher
Motorized escape and refocus mechanism (coarse)
Coarse/fine switchable


Motorized 6-position objective revolver
ref:TI-ND6-PFS

Motorized 6-position reflector turret

Perfect Focus System with PFS offset dial

Eyepieces

CFI 10X/22

Eyepiece tube
ref:TI-TS

Eyepiece Tube Base Unit
ref:TI-T-B

Transmitted Illumination

IDiascopic Illumination Pillar 100W
ref: TI-DH-612504

Precentered Dia illumination lamphouse
ref: D-LH/LC-256834

V2-A LL Halogen Lamp bulb 12V 100W

Stagetop Incubator System

Manufacturer: Oko-Lab

Heated Chamber for Nikon Motorized Stage
ref: H301-NIKON-Ti-S-ER

All in One Controller with C2 Air Mixer
ref: UNO-T-H-CO

4x 35mm Dish Holder
ref: 4×35-M

96 Well Plate Holder
ref: 96MW

#1 1×3 inch Slide and #2 35mm Dish Holder
ref: GS35-M

1×2 inch Chambered Cover Glass Holder Magnetic
ref: 1xLABTEK-M

1×3 inch Chamber Slide Holder Magnetic
ref: 2xGS-M

Open frame for multi well plates w/ oil immersion objectives
ref: MW-OIL

Nikon C2+ point scanning
Scan Head

Model: C2+ point scanning
Manufacturer: Nikon
ref: C2-SH-42065

Laser unit

ref:LU-N4-SN40475

– 405nm – 15 mW
laser head

– 488nm – 15 mW
laser head

– 561nm – 15 mW
laser head

– 640nm – 15 mW
laser head

Detectors

ref: C2-Du3-14487

3 confocal PMT (2 filter cubes)

– C2 V4C EM 445/35 + 660LP
ref: Chroma 99430/C171408

– C2 EM 525/50 + 600/50
ref: Chroma 99441/C172339


1 T-PMT

Computer
Advanced Imaging Workstation

Ref: 77019756

Hp Z4

Intel Xeon 3.7 GHz processor (8 cores)

464GB DDR4 2666Mhz RAM

NVIDIA RTX4000 8GB

512GB m.2 Turbo SSD

1TB M.2 Turbo SSD data drive

12TB data storage

Window 10 64-bit professional

37.5 inch IPS, LED backlit monitor

Software

Nikon NIS elements AR 5.41.02 (buid 1711)

Suitable for Techniques

Live Imaging

3D imaging

Multi position acquisition

Large area tile/mosaic scans

Optical sectioning

Nikon Objectives
CFI Plan Apochromat Lambda D 4X/0.2

Ref: MRD 70040

Immersion: Dry

Collar: none

Coverslip Thickness: 0/0.17 mm

Working Distance: 20.0 mm

Field of View: 25mm

Tube Length: infinity

CFI Plan Apochromat Lambda D 10X/0.45

Iref: MRD 70170

Immersion: Dry

Collar: none

Coverslip Thickness: 0.17 mm

Working Distance: 4.0 mm

Field of View : 25 mm

Tube Length: infinity

CFI Plan Apochromat Lambda 20X/0.75

ref: MRD00205

Immersion: Dry

Collar: none

Coverslip Thickness: 0.17 mm

Working Distance: 1.0 mm

DIC slider: N2

Field of View: 1.25 mm

Tube Length: infinity

Coverslip Thickness: 0.17

λ : Nano Crystal Coat

CFI Plan Apochromat 40X/0.95

ref: MRD00405

Immersion: Dry

Collar: yes

Coverslip Thickness: 0.11-0.23 mm

Working Distance: 0.25-0.17 mm

DIC slider: M/N2

Field of View: 25 mm

Tube Length: infinity

CFI Plan Fluor 40X/1.30 Oil

ref: MRH01401

Immersion: Oil

Collar: no

Coverslip Thickness: 0.17 mm

Working Distance: 0.24 mm

DIC slider: N2

Field of View: 0.63 mm

Tube Length: infinity

CFI Apochromat Lambda S 60X/1.40 Oil

ref: MRD71600

Immersion: Oil

Collar: none

Coverslip Thickness: 0.17 mm

Working Distance: 0.14 mm

Field of View: 0.37 mm

Nomarski Prism: N2

Tube Length: infinity

CFI Apo LWD Lambda S 40X/1.15 W

ref: MRD77410

Immersion: Water

Collar: yes

Coverslip Thickness: 0.15-0.19 mm

Working Distance: 0.61–0.59 mm

Field of View: 25 mm

Nomarski Prism: N2

Tube Length: infinity

Bright field imaging
Condenser

Ref TI-C-518109

Long workind distance Condenser lens ref:TI-C-LWD

NA: 0.52

WD: 30 mm

Manual

TC-C-DICP DIC Polarizer

Ti-A Analyzer Block Installs in Epi Fluor Turret

DIC accessories

T-C DIC Module LWD N2 Dry
T-C DIC Module LWD NR Dry

D-C DIC Slider 20
D-C DIC Slider 40x I
DIC Slider 60x I-RD-C
DIC Slider 100x II

Fluorescence
Illumination

X-Cite 120 LED Boost

Manufacturer: Excelitas Technologies
Catalog Number: XT120L

Filter sets
DAPI/Hoechst/Alexa Fluor 350

ref: Nikon 96221 / Chroma 39000

Excitation: AT375/28x
Mirror: AT415-DC
Emission: AT460/50m

EYFP/Venus/Citrine

ref: Nikon 96227 / Chroma 39003

Excitation: AT480/30x
Mirror: AT505DC
Emission: AT535/40m

Texas Red/mCherry/Alexa Fluor 594

ref: Nikon 96231 / Chroma 39010

Excitation: AT560/40x
Mirror: AT600DC
Emission: AT635/60

Stage

Motorized Stage with Encoder
Manufacturer: Nikon
ref: Ti-S-ER

Stage Joystic:
Manufacturer: Nikon
ref: TI-S-E JOY-559960

Adapter for Oko-Lab Stagetop Incubator System
ref:H301-NIKON-TI-S-ER

Yokogawa CSU-W1 spinning-disk confocal
Laser unit

LUNF-XL laser combiner
ref:77098033

– 405nm – 50 mW
laser head

– 445nm – 25 mW
laser head

– 488nm – 60 mW
laser head

– 514nm – 35 mW
laser head

– 561nm – 50mW
laser head

– 594nm – 40 mW
laser head

– 640nm – 40 mW
laser head

– 405nm – 50 mW for photo-manipulation

Camera

Manufacturer: Andor

Model: Zyla 4.2
ref: ZYLA-4.2-USB3

Sensor type: Scientific CMOS

Sensor size: 13.3*13.3 mm

Pixel count: 2048(H) × 2048(V) = 4 MP

Pixel pitch: 6.5 μm (H) x 6.5 μm (V)

Bit depth: 12 bits and 16 bits

Quantum efficiency: 72% around 600nm

Max Frame rate: 128*128: 1578 fps

Scan Head

Model: CSU-W1 spinning-disk confocal
Manufacturer: Yokogawa
ref: CSUW1-T1N12-C10C00-F2ED

Dichroic mirrors and Filters EM wheel

Dichroic mirrors

DM 405/488/561/640 dichroic beamsplitter
Manufacturer: yokogawa
ref: nikon MHE46420

DM 445/514/594 dichroic beamsplitter
Manufacturer: semrock
ref: nikon 99226

Emission filters
DAPI- EGFP – AF568/mcherry – AF 647

Manufacturer: semrock
BrightLine® quad-band bandpass filter – 440/521/607/700 nm
ref: FF01-440/521/607/700

DAPI BP

Manufacturer: chroma
ET436/20x single-band bandpass filter
ref: ET436/20x

ECFP BP

Manufacturer: semrock
482/25 nm BrightLine® single-band bandpass filter
ref: FF01-482/25

EGFP BP

Manufacturer: semrock
525/50 nm BrightLine® single-band bandpass filter
ref: FF01-525/50

EYFP BP

Manufacturer: Chroma
ET545/25x bandpass filter
ref: ET545/25x

AF568 BP

Manufacturer: Chroma
ET605/52m bandpass filter
ref: ET605/52m

AF568 BP Extended

Manufacturer: semrock
617/73 nm BrightLine® single-band bandpass filter
ref: FF01-617/73

mCherry BP Extended

Manufacturer: semrock
641/75 nm BrightLine® single-band bandpass filter
ref: FF02-641/75

AF647 BP

Manufacturer: Chroma
ET705/72m single-band bandpass filter
ref: ET705/72m

Scanning Method

Microlens‐enhanced Nipkow disk scanning

Maximum Disk Speed

Max 4,000 RPM
200 FPS

Disk Unit

50um or 25um (up to 2) motorized

Field of View

17x16mm maximum

Illustration of the Stagetop Incubator System
GS35-M
4X35-M
1xLABTEK-M
96MW
2xGS-M

MW-OIL

C2+ filter scan head
Emission filters for the C2+ scan head (peel off 445/35 +660LP) for DAPI and Alexa 647 and band pass filter (525/50 +600/50) for eGFP and mCherry/Alexa fluor 568
CSU-W1 emission filters

Contact fbonnet@mdibl.org today to learn more about our services and how we can help you achieve your research goals. We look forward to working with you and unlocking the mysteries of the microscopic world together!